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Seminar 877  
Date:2017-09-20
Update:2017-11-07

  

Seminar:  877  

1

Speaker(s)

Yu, Chaofang

Topic

Study on Substitution Method for Efficacy Evaluation of Egg Yolk Antibody against Riemerella anatipestifer Infection

Abstract

For the efficacy test of animal biologics, most vaccines and antibody biologics are evaluated by challenging target animals, which is a more direct and accurate manifestation of immune response in animals. The study aims to find the correlation between challenging protections and sera IgY titers in ducks in order to replace the current challenging ducks (in vivo) method with sera IgY titers detection (in vitro) method for efficacy evaluation of bivalent egg yolk antibody against Riemerella anatipestifer infection. In addition, for those anti-RA IgY products failing to evaluate the efficacy with challenge animals, the detection of sera IgY titers may serve as an alternative for efficacy assessment. The 3Rs principles goal could be achieve gradually by reduction in the number of laboratory animals used.

2

Speaker(s)

Lu-Jen Ting

Topic

Constructing a plasmid DNA as differentiable positive control for PCR

Abstract

Polymerase chain reaction (PCR) has been introduced into diagnostic laboratories as a routine for detecting etiological agents of infectious diseases. When employing this method, one of the considerations is the false positivity of PCR results. To minimize the risk of producing false-positive results, nucleic acid for positive control is a factor need to be take care of, in addition to reagents, equipment, and working areas. The aim of this study was to construct a DNA as differentiable positive control for Neospora PCR test. The positive control is a plasmid inserted with a fragment composed of an inner sequence of avian influenza virus and the primer annealing regions of Neospora sequences at both ends.  When the construct was employed in the Neospora-AI PCR, the reaction can generate a product 211 bp longer than that of the normal Neospora positive PCR product. In addition, the product can be identified by nested PCR which is used for the routine identification of avian influenza viruses. In addition, the same strategy was used to construct the Parainfluenza 3-goatpox differentiable positive control DNA, followed by transcribing into RNA with the MEGAscript™ SP6 Kit.