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Seminar 819  

Seminar:  819

1

Speaker(s)

Shu-Hwae LEE

Topic

Surveillance and monitoring on bovine spongiform encephalopathy (BSE) in Taiwan and current status of BSE control in Canada

Abstract

The laboratory standard operating procedures (SOPs) for BSE have been established (refer to Annex 15) based on the Terrestrial Manual. Brain stem of each BSE suspected cattle was collected and longitudinally dissected from midline into two parts. One part of the sample was frozen at -20 freezer, and another part was fixed in 10% neutral formalin solution and examined by routine histopathological examination specially for spongiform changes, immunohistochemistry (IHC) for detecting any accumulations of abnormal prion, or by immunoblotting and other enzyme immunoassay to detect the presence of protease-resistant prion protein. Commercial kits including Prionics®-Check Western kit, Prionics®-Check LIA kit, BIO-RAD PLATELIA® BSE purification and detection kit (Bio-RAD TeSeETM purification and detection kit) are used in the surveillance system. Initially, all samples were tested by histopathology and ELISA method to look for any suspicious samples. Final confirmation was made by the result of IHC and immunoblotting. The overall surveillance comprises both general and targeted surveillance. General surveillance focuses on examination of cattle brains randomly sampled from slaughterhouses and farms under movement control for tuberculosis. Active surveillance has been carried out since 1998 and a total of 7,698 bovine brains have been examined for BSE from 1998 to 2012. All samples tested were negative for BSE. More than 99% of the samples originated from the cattle over 24 months of age. The data obtained reveal Taiwan is still free of BSE.

I have collected and learned the current control measures for BSE in Canada in the visiting to Canada from 8-20 September, 2011, including the mission of Canadian Food Safety Bureau, BSE risk analysis and monitoring methods, cattle carcass inspection process and the specific risks material removal methods, standardized feed ban. And I also visited OIE BSE reference Laboratory. These data and experiences are quite valuable and available for the intended reference of the preventive strategy of bovine spongiform encephalopathy (BSE) in the future in Taiwan.

2

Speaker(s)

Chien Tu

Topic

Development of a fish cell line and preliminary characterization of koi herpesvirus isolated in Taiwan

Abstract

A cell line (KoG) from the gill of koi, Cyprinus carpio, was developed and subcultured more than 30 passages in 2012. The morphology of KoG was fibroblast and epithelial-like mixture cultured in L-15 medium supplemented with 10% fetal calf serum. Chromosome analysis showed that 31% of the KoG cells maintained the diploid chromosome number (2n= 43) at passage 30. KoG cells infected with KHV revealed cytopathic effects (CPE), such as vacuolation in cytoplasm, syncytia, cluster of rounding cells. The titer of KHV propagated in KoG was 105.6-10 6.1TCID50/ml. KHV were further confirmed by indirect immunefluorescence assay, electron microscopy and major gene sequence analysis. According to the sequence analysis of two variable regions (marker 1) and (marker 2), Taiwanese isolate is consistent with Japanese linage.