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Publication Detection of Newcastle disease virus isolates in Taiwan using the re...  

Update Date: [2013-11-13]

NO.: 34
 
Reportno.

AHRI report No.34

Topic

Detection of Newcastle disease virus isolates in Taiwan using the reverse transcription-polymerase chain reaction

 

Department

1.Dep3rtrnent of Veterinary M&icine, National Taiwan University, Tairei, Taiwan 1%ROC
2.Aninal Health Research Institute, Council of Agriculture

Author Hsiang-Jung TSAI 1 9JthFa LIN 2
Summary

Various Newcastle disease (ND) vxination programs were tested for their proeAon efficacy. fri trial 1, SI>? chicks were vaccinated with an attenuatul or inactivated ND vaccine at 4-thy-old, then bxtJ again with an attenuated or inactivatedND vaccineat7days ofageor 14 days of age. Allvaccinatulgroups showed a good protection rate (80-1(0%) when they were challenged with a ‘&ulent ND Nirus at 5-wk-okI. In trials 2 and 3, several groups of broilers with high maternal antibodies (geometric mean hemagglutination-inhibition antibody titer was 1:84,5-
1:135 at 4-thy-old) were simultaneously immuthed with an attenuati and an inactivated ND vaccine at 4 days of age, and then revaccinated with an attenuated ND vaccine at 17-thy-old. Other groups of broilers were first vaccina1 with a live vaccine at 4-day-old, revaccinated with an inactivated vaccine at 14-thy-old, and then bxsted againwithalivevaccineat 17-thy-olcL Gcxdproationrate(80-1CO%) was achieved in both groups when they were challenged at 5-wek-old. When they were challenged at 3-wd-o1d and 4-wd-old, the latter broiler groups had a significantly higher protuotion rate (1(0%) than the formal broiler groups (50-80%) (p4105).

Keyword

Newcastle disease(ND);Vaccination proj amsProtection efficacy