Home
 /  Publication  /  Volum 39  /  Publication Examination of Meat Components in Commercial Dog and Cat F...
Publication Examination of Meat Components in Commercial Dog and Cat Feed by Usi...  

Update Date: [2013-11-13]

NO.: 39
 
Reportno.

AHRI report No.39

Topic

Examination of Meat Components in Commercial Dog and Cat Feed by Using Polymerase Chain Reaction-Restriction Length Polymorphisms(PCR-RFLPs)Technique

 

Department

1.Dearurient of Vetinary me3icineCoUege of Vexinary Mdicine, National Chur*g-Hsiug University
2.Animal Health Research listitate, Council of Agriculture

Author Hsien-Chi WANG1 Shu-Hwae LEE2;Tien-Jye CHANG1 and M114-Uang WONG1
Summary

It has keen shown that certain slow neurological diseases swzh as bovine spDngiform encephalothy (also known as “mad cow” disease) cxnild It transmittal through contaminatd focd intake by animals; therefore, the examination of meat compcnent in com-mercial fJs is impDrtant for the control of the disease in public health. The combination of poilymerase chain reaction- restriction frag-ment length polymorphisms (PCR-RFLPs) tohnirpe was applied to examine the meat compo’nenls in dog and cat u.minaiy courercial f3s The priial nucleotide souence (359bp) of animal mitcxhondrial cybochrome b (cyt b, CY1) gene was amplified by PCR and then digestd with restriction enzyme Alu I or Mko I. hi this book eight brands of commercial dog and cat fis available in Taiwan were examined. All hands of dog f-Js that were testl contained meat from four different animals (cattle, pig, gout and chictn). In cat f-Js, the chicken meat was found in five out of eight hands.

 

Keyword

BSEoyt b gene$og and cat commercial diet9CRpAymorphism.