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Publication Ultrastructal Localization of Virus Antigen Using an Immunogold Meth...  

Update Date: [2013-11-11]

NO.: 26
 
Reportno.

AHRI report No.26

Topic

Ultrastructal Localization of Virus Antigen Using an Immunogold Method:Pseudorabies

Department

Taiwan Animal Health Research Institute

Author N.J. Li;Y.P. Lin;W.C. Ho
Summary

Cdloidal gold imsnuno electron inicrosopy Las keex employed s sthfical1y clett roseuriceNes vims antigen in negatively stained srimens. This rapid and sensitive iminuncessay was found to 1e applicable to rtia1ly purified vinis pxention.
Pseudorabies virus antigen were lakeled in ultrathin section of RK1 3 cell, using incbxect immunogold methcd. Antigens were tagged ‘thth Snm, pr 1 Snm, Gold rortic)es,the gold particles were aesent on viral nudexapsid and viral envelores. Ininunogold stain of ultrathin sections can fried anklitional information of virus-licet cell interactions.
 

Keyword

Pseudorabies; electron micrcGcopy;linmunogold Methcd