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Publication Establishment of a Rapid Method in Cell Culture to Isolate Mycobacte...  

Update Date: [2013-11-13]

NO.: 36
 
Reportno.

AHRI report No.36

Topic

Establishment of a Rapid Method in Cell Culture to Isolate Mycobacteria

 

Department

Animal Health Research Institut Council of Agxicultuxe Exutiw Yuan

Author Lin,King-Fu,J.F.SY.S.Wu,W.M,Chajzgandj.RChiau
Summary

Atotalof 391 isolates of my ctexiuxnbvis CM. bovis) and 1Oiso1a of inyco1cterium avium complex (M. avium complex) were obtainod from 631 head of catt1e 175 deer and 88 gcet with pccitive reactions by PPD (purified rrotein derivative) sian test. The Marc-145 cell line was used to culture mycobtcleria. Aft 9SUInJnafl thne essages(9-P) in Marc-145 cells, the YL strain of M. bovis was incculrni in slant for the add-fast microorganism count. These results showed that the 9-PM bovis-YL stain proliferated well in Marc- 145 cells. This method would improve the culture gcddure of mycolateria.

 

Keyword

mycokecerium bovs(M. bovis), mycobtcterium avium complex(M. aviurn complex)