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Publication CHARACTERIZATION OF LIPOPOLYSACCHARIDE EXTRACTED FROM HAEMOPHILUS PL...  

Update Date: [2013-11-11]

NO.: 26
 
Reportno.

AHRI report No.26

Topic

CHARACTERIZATION OF LIPOPOLYSACCHARIDE EXTRACTED FROM HAEMOPHILUS PLEUROPNEUMONIAE

Department

1.Taiwan Animal Health Research Institute
2.Veterinary Medicine Department, National Taiwan University

Author W.M.Changl*.Y.YanglS.S.Lai2
Summary

The pheno1-exnDi lipopolys&xhariit (LI’S) gave a pc6itive reaction bth in L.imulus anbce&cyte lysate (LAL) t and derroal Shwarizrnan reaction, but not in hernolysis test. The SDS-PAGE ratten of exixacted LI’S revealed by silver stain showed a laMer form and was considered as a smcoth type of LPS ttm, The LD of LI’S was 8.9mg n mice when Wraeritoneally inj5ti ‘dth D-galactcGaxnine. It was also found that the soluble antigens of HP contained large amounts of LPS antigen. Two immunoprecipitation lines of LI’S which were serotype-sthfic were observerl when antiserum against homologus serotye of HP was used in double immuncdiffusion test. huiratracheal incculation of LI’S into gainesa pigs inducer! congestion adn lniorrhage of lung. Histojethologicafly, extensive honchopneumonia and aggregation of spindle, pykno& cells were observed. The immunogenthty of LI’S was tesi by using mouse proation test. The exrerimental data indicateed that LI’S antigen game a good protective rate.
 

Keyword

hpopolysaccharkles; Limulus amboebccyte lysate -325 LAL