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Publication Determination of diethylstilbestrol in feed by high performance liqu...  

Update Date: [2013-11-14]

NO.: 41
 
Reportno.

AHRI report No.41

Topic

Determination of diethylstilbestrol in feed by high performance liquid chromatography

 

Department

Animal Drugs lnsection Branch, Animal Health Research lnstimte Council of epartmen Ex&zutive Yuan

Author S.FChen, CH Liii, MY Kuo, J.FSu Shin-Ru Yeh, Chia-Chen Chang, Tien-Lai Hsu, HsimJu (lao, Wen-Hung Chin, U ° Chien-Qith Wu and Mau-Jinn Kwaug
Summary

High performance liquid chromatography equippal with photodiode array dettor (HPLQPDA) was usel to measure the quantity of diethylstilkestrol in f4. To prevent the false pcthve reaction causal by low oDnceniration of diethyistilkestrol in feel, high performance liquid chromatography epilppol with mass spaztophotometer (HPLQMS) was further usel as a omfirmation tst. The samples were prepared by stirring with methanol, followed by a centrifugation step and then an evaporation step, uinmaiy The residual is then dissolved with 20% methanol and the aajt’cms solution is cleaned by the assay of solid-phase extraction (SPE) using OasisRHLB to remove the matrix intrfexenc and the eluate was obtained and analyrd by HPLQPDC or HPLQMS. The result showed that the average rwver rats of diethylstilirstol in the spiked samples were during the range of 80.8 - 107.5%. The Limit of DetoDtion (LOD) of PDA was 0.03 ppm and the IDD was 00)5 ppm when the MS was used.

Keyword

High Performance Liquid Chromatography, Diethylstikesirol, Fl