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Publication Assessment on Screening and Differential Detection System for Catego...  

Update Date: [2013-11-14]

NO.: 41
 
Reportno.

AHRI report No.41

Topic

Assessment on Screening and Differential Detection System for Categorized Antimicrobial Substance Residues

 

Department 1.Animal Health Research fristitute, Council of Agriculture, Executive Yuan
2.Bureau of Animal and Plant Health Inspection and Quarantine, Council of Department Agriculture, Executive Yuan, Taipei, Taiwan.
3.Graduate Institute of Biotechnology, Chinese Culture University, Taipei, Taiwan.
4.Deperiment of Food Science, National Taiwan Ocean University; Keelung, Taiwan.
Author LinW. Hi., LI. Went, CF. Chang3, CM. Chang4, Hi Sung2., P. H. Chaol
Summary

The dettion of antibiotics residue is generally analyaed by the growth inhibition ring of sensitive microbial stains revealed on agar plate diffusion test. An integratl efficient detection system of initial screening detztion and suteuent differential detztion is performed in this research report The initial screening detection adopts microbial gel coloring method (MGCM) with permitted commercial peckage on antibiotic residue detztion over various animal prcducts. The PremiRTest kit by Dutli DSM food comny effectively covers 9 antibiotics classes with 34 drugs of routine usage including ‘ceta-lans, tetr&Dyclines, aminoglyckJes, and sulfa drugs. Our detection result with PremiRTest kit revealed superior detection sensitivity on antibiotic residue classes as tote more sensitive than allowed maximal residue quantity of government regulation. Moreover, the suteuent differential detection adopts four plate test (FF1’) with permitted microbial media and sensitive microbial stains on antibiotic residue dettion over various animal products. Merck antibiotic test agars (MATA) are respectively applied on four plates with catalog numters of[I) 1(1563 pH 6.Q [II] 1052’?OpH 7.2, [III] 1(1564 pH 80, and [P/) 1(15&I pH 8.0 in which platz ft
lHhl]fl2IY 11,111) are incculatl with Barillus subtilis ATOD 6633 and plate [IV] with Sarcina
lutz ATOD 9341. Screening and differential detection among several routine antibiotics classes can te accomplished by respective sensitivity ranking pattern of antibiotic residue with FPT inhibitory ring s& including teta-lacms [P1>1>111>11 (or II> III)], tetracyclines [1>11>111 >IV], aminoglycc6ides [III >11>1 >IV), macrolides [IV >111>11 >1).
fri conclusion, the integrated detztion system of initial screening detection and suteuent differential detztion may te evidently efficient in fulfilling practical goal of antibiotic residue deetion over various animal products. Initial screening detztion of MACM may te established as voluntary pre-dettion by fcxxl product suppliers. Suhcequent differential detuztion of FPT may te established as surveillance detztion as of integrated detztion system by regulatory institutions. Methodology performance of test detection limit is effectively within allowed veterinary drug residue limit supervised by the Department of Health which in bun efficiently assures regulatory compliance on legal standards in allowed maximal antibiotics residue quantity.

Keyword

antimicrobials residue; microbiological method; screening; differential; microbial gel coloring method; four plate method.