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Publication Development of Enzyme-Linked Immunosorbent Assay (ELISA) for Detecti...  

Update Date: [2013-11-11]

NO.: 27
 
Reportno.

AHRI report No.27

Topic

Development of Enzyme-Linked Immunosorbent Assay (ELISA) for Detecting Eperythrozoon suis Antibody in Swine.

Department

Taiwan Animal Health Research lnstitu The Branch Institut of Animal Drugs eparbu

Author 3.F. Su;W.C Hwang
Summary

Thx native bactrins against Pasnxefla multcida (PM) were snd for pctncy in chicks and ducldings. The sensitivity in dudding challenged by live PM was the highest. Followed by chick and then mouse. The 50% lethal dc6e (UDSO) of the group challenged with standard strain of PM were 10-8,24 (duckling), 10-7.42 (Click) and 10-6.75 (mouse); and that of the group challrangftl with field stain of PM was 10-8b1 (duckling). 10-7.68 (chick) and 10-7.16 (mouse), resaDtive1y. Above result shows that the toxicity of field strain of Pm is slighily higher than standaid strain of PM. After the trials of three commercial lecterins, it seemed that the mouse was the kest sutiable among 3 excerimental animals in its stability and cxt for the flncy tat. The protDtive index of mice should Ic larger than 0.8 by using the n-otative index to suItitue the current standard of insceDtion. The effetive storage time of above kacerins is less than6months. So. Wwewanttopro1ongtheespintiontim the lest is to improve the quality of the lecterin itself.

Keyword

EiSA; Enzyme-Linked ImmuncGorkent Assay ; Paseurella multocida