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Publication Detection of Salmonella by multiplex polymerase chain reaction  

Update Date: [2013-11-14]

NO.: 41
 
Reportno.

AHRI report No.41

Topic

Detection of Salmonella by multiplex polymerase chain reaction

 

Department 1 Animal Health Research histitute, Council of Agriculture, Executive Yuan
2.Deertment of Veterinary Merlicine, National Pingtung University of Science and Technology
Author Chang, WM 1, MH Liao2, KFLin 1, SM Huang 1, JH Kuo 1 ,JRShiau 1
Summary

A multiplex polymerase chain reaction (rnPCR) assay, using two sets of raners designed acoording to the srences of invA and 152(I) genes, was developed for the identification of Salmonella, The rnPCR assay was evaluai by using 12 sJ strains and 251 isolaal stains, which were atthbuei to more than 23 different serovars of group A, B, Cl, C2. Dl, D2, El and E2. All stain esJ, exce 2possessoi z4 complex (H antigen) stains, were po€itive in the mPCR assay. The mFCR assay was compared ‘nth the iraditional culture and biological characteristics to determine the sthficity and sensitivity of the test. Using a total of 450 swine fecal samples collecei from slaughterhouse during 9 months, 49 samples were identified to lie positive in both the mPCR assay and the traditional culture and biological characteristics. hi addition, 50 duck fex:al samples collectl from animal house of duck were also tested, and all were found to lie negative in both assays. Thus, the mFCR assay developed can e actically used as an auxiliary step for identifying Salmonella.

Keyword

Salmonella, multiplex polymerase chain reaction, diagnosis