Home
 /  Publication  /  Volum 35  /  Publication Development the method of isolation of mycobacteria in tis...
Publication Development the method of isolation of mycobacteria in tissue cultur...  

Update Date: [2013-11-13]

NO.: 35
 
Reportno.

AHRI report No.35

Topic

Development the method of isolation of mycobacteria in tissue culture

 

Department

Animal Health Research hstibite, Council of Agriculture.

Author Lb. KingFu*C.C Yung$.Y Le).R hiau;Y.S Wu;Wei-Ming Chang$.C ChenJ.F su
Summary

developaisolationmeth&ofmycolnriabytissuecultuxes, the isolation strain of rnycoltium bis-M,is YL strain was inDthl in the tissue cultures ofswinealvkrmacrophages (SAM),Marc-145 andOOD, respaiively,The result revealed that theM. bcvis-YL stain was found growth well in the alxwe mention tissue cultures at 3 day afr incoilation,
Summary The srthmens cx)flecting from the rc€itive reaction cattle by PPD(Purifioi pit derivative) incoilating in slant Qnensein-Jensen maliurri,L-J M) and Marc- 145 cell Erie, reectively, forisolatingtheM.bovis .Theresult ofthealrovetestreveablthat theM, bcvis was isolatlat7to 14 days aftrincculatii*gthMarc-145 cellhines?and isolatlat4 to 15 weeks aftculturir*ginsknt.Bothmethcds of isolating rae were as same as 43.46%(33/76)..
 

Keyword

flcMycohacterium isM. bisSwine alveolar macrophages SAMSbnt medium
(Lowensein-Jensen indium, L-JM).