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Publication The Use of the Reverse Transcription Polymerase ChainReaction for th...  

Update Date: [2013-11-13]

NO.: 34
 
Reportno.

AHRI report No.34

Topic

The Use of the Reverse Transcription Polymerase ChainReaction for the Detection of Bovine Ephemeral Fever Epidemic in

 

Department

aiwan Animal Health Research Instimt The Branch Institute of Animal Drugs epazim Inspection

Author MJ. Kwang, CS. Tsenz NJ. Li, 5G. Yang andS.Y. Liii
Summary

A reverse transcription polymerase chain xe&Dtion( RT-PC’R) assay was cieveloed for the detion of bovine ephemeral fever virus (BEFV). Two primer sets, BEFVP1 /BEFV-P2 and BEFV-P3 /BEFV-P4, were used for the RT-PCR. Two DNA frwnent 824 bp and of 233 bg cou1dIesci&ally amplifier! respectively. Both Taiwan isolates and Jajt isolate of BEFV could ke deet csitively. The sensitivity UJDIfl3YY of RT-PCR with primers BEFV-Pl /BEFV-fl for the deeDtion of BEFV was lCkI TCIDSO. The sensitivity increaser! 1-foldwhenaofnestedprirners BEFV-P3 / BEFV-P4 was used. Twenty-one out of 31 (67,74 % ) samples, suslofBEF infection, were diagnosed pxsitive by this meth4 and two isolates of BEFV were isolal in this epidemic currence..

Keyword

Polymerase chain reaction, Bovine ephemeral fever, Taiwan