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Publication Isolation of Infectious Bovine Rhinotracheitis Birus from Calves wit...  

Update Date: [2013-11-04]

NO.: 12
 
Reportno.

AHRI report No.12

Topic

Isolation of Infectious Bovine Rhinotracheitis Birus from Calves with Respiratory Disease
 

Department Taiwan Animal Health Research bstitu
Author K.F. Lin T.C. Chiu; Y.S. Lu; I.P. Chan
Summary

One stain of infectious bovine rhiontracheitis was isolal from calves with respiratory disease in bovine kidney cell cultures, and purified by plaque forming method, The virus replication was inhibited in the esent of 5iodo2Ldecxyuridine. It was sb1eatPH?to 8, but icees activitybelowPH5, itwas inactivaibyexpceureat 561E2 for 3(Y, and at EO1D for 13 and destroyed by trealment of 1 M, MgCI2 at 5CftEj, for 30.

The isolated virus was serologically related to the cxz’ocer strain of infectious bovine rhinofracheitis, It did not agglutinat bovine gcet and shp red blood cell at 4t. The piecipitin line appeared when anti- IBR-C rabbit serum wasallow to diffuse toward the rimary iso1aI virus, but anti-B VD-NADL or anti-P13-SF-4 rabbit serum did not react to the iso1ai virus.

The isolated virus was serologically related to the oooper strain of infectious bovine rhinotracheitis, It did not agglutinat bovine, gcet and shp red blood cell at 4t. The uecipitin line appeared when anti- IBR-C rabbit serum wasallow to diffuse toward the isolai virus, but anti-B VD-NAt)L or anti-P13-SF-4 rabbit serum did not react to the iso1ai virus.

The isolated virus prodwedcytopathiceffectinBT, BK, AuBEK, ESK, PK-15, and HeLa cell cultures.

Keyword

Bovine Rhinotracheitis Dims Respiratory Disease