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Publication Comparison of Actinobacillus pleuropneumoniae RTX-toxin I gene (apxI...  

Update Date: [2013-11-13]

NO.: 33
 
Reportno.

AHRI report No.33

Topic

Comparison of Actinobacillus pleuropneumoniae RTX-toxin I gene (apxI) among serotypes by Restriction Fragment Length Polymorphism

 

Department

1.Anirriai Health Research fristitie, Council of Agricuiftxe 
2.Depea-tment of Vetrinary Mdidne, National Taiwan University

Author W.M. Changl Ui2S.C Yangl ;Y.S Wul J.R. Shiaul
Summary

Four piroers &xording to the sotnce of activator gene (apx IQ and structuxai gene (apxlA) of ApxI were synthes&{ and used for dyrrierase chain reaction (PCR) to amplify srei& DNA fragment among 12 Ap seroty, The resuit indicated that serotye 1,5,9, lOand 11 h&JaneilengthofamplifibiDNAfragmentwith3 iroers. However, serotype 1,9 and 11 had also obtained a particular length with the other palmer &xording to the sepienceof apx ICA gene. The stnces ketween 29?4 and 3011 of apx IA of serotye 5,10 det&toi by PCR were found different with those nmmary of serotype 1,9 and 11. The FCR amplified 3.9kb DNA fragment ofapxlCA from serotype 1,5,9,10 and 11 were analy2rd by digestion with 10 restriction enzymes. The Hind77NPBdll?and ApeLI cuttng si of apx IA of serotype 1,9 and 11 were different from those of serotype 5 and 10. The restriction map of the 3 kb DNA fragment of apx IA amplified from the kcal isolate of serotype 1, were identai to those rextJ geviously. Chang WM and LeA SS. Comparison of Actinokeillus pleuropneumoni R’IX-toxin I gene (apxl) among serotypes.

 

Keyword

Actinokthllus pleuxopneumonrpxl.