Home
 /  Publication  /  Volum 23  /  Publication DETECTION OF THE ANTIBODIES AGAINST GOOSE PARVOVIRUS BY AN...
Publication DETECTION OF THE ANTIBODIES AGAINST GOOSE PARVOVIRUS BY AN ENZYME-LI...  

Update Date: [2013-11-05]

NO.: 23
 
Reportno.

AHRI report No.23

Topic

DETECTION OF THE ANTIBODIES AGAINST GOOSE PARVOVIRUS BY AN ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

Department

Taiwan Animal Health Research Institute

Author MC. KwangHJ. Tsai;YS. LuAndiewC.Y. Fei;Y.L. LejJ.F, Un$Dift
Summary

An indirect enzyme-linked rnunor1:ent assay (ELLSA), used a crick antgen, was develorei to cleat anti&dies against gxe rvo’’inis in gcose senim samples. Thekestliwasobtainedbyugl:1OOdiluitJsenimsampl, lOugknlrabbit anti-gocee IgG, 1:5(0 diluti reroxidase lakelled gcat ann-rabbit IgG, and by inculatl th subtratfor 30m n. Ev uat! by the&.LSA, it was found that both thegoc6e incculael with the attnuat! c inactivael gocGe rervovinis vaccine gave good UflUflI, antibody respnse, and the antibcdy na could last at least 8 to 16 welc. Forty-six senirri samples collect flora chungh area in 1981 were found negative in ELISA antibody; however, 3 out of 14 seryrn samples collect! fcrnn Kaohsiung county in 1986 were found pc6itive ELISA antibody against gcxte cervoviius. The ELISA was found to ke more sensitive than agar gei prtiprnn est in cleection of antibodies 4ganst goc€e p3rvovrus
 

Keyword

ELISA; goc€e j:ervovinis ; cleat antibcziie