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Publication Establishment of an electron microscopic method for the dctection of...  

Update Date: [2013-11-13]

NO.: 31
 
Reportno.

AHRI report No.31

Topic

Establishment of an electron microscopic method for the dctection of procine reproductive and respiratory sydrome virus

 

Department

Taiwan Animal Health Research Institute

Author N.J. Li*;Y,P. UnS.Y. (Then
Summary

An outbreak of dne repductive and resgiatory syndrome (PRRS) was diagnosed in the pig faa-n iccatul in northern Taiwan. A cytopathic virus was isolated from the lung. lynph ncde and spln of affected pigs using alveolar macrophages of primary seDif ic-pathogen fift piglets, The swine alveolar macrophages (SAM) were
Summary degenerated and lysed on the sa:ond and third days pDGt thcculation. Examination of the infected SAM by transmission e]ec- ton microscope revealed viral particles within the dilated rough endoplasrnic reticulum and cytoplasmic vaculces. Viral particles were observed from the suppernatant of PRRSV thfecnd SAM monolayer by the phosphotungstic salt negative stain.

 

Keyword

Negative stainthin sectionprccine xc cthrtiverespixatory syndrome.