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Publication The Establishment on Diagnostic Nucleic Acid Probe for Egg Drop Synd...  

Update Date: [2013-11-13]

NO.: 34
 
Reportno.

AHRI report No.34

Topic

The Establishment on Diagnostic Nucleic Acid Probe for Egg Drop Syndrome Virus in Taiwan Isolates

 

Department

1 Taiwan Animal Health Research Institute
2.Dertrnent of Veterinary Micine National Taiwan University

Author Der-Tyan Lini, Shiow-Sney Lai2, Tien-Lai Hsul, Jei-Fu Sul, Shth-Yuh Lini
Summary

In cder to esblisli the diagnostic rndeic acid prol for detertg to egg drop syndrome virus (EDSV) DNA, the genome of EDSV GD strain isolated from chicken oviduct was clon& in pElueSoript II KS (+1-) vertor. Eight DNA fragments in EDSV were clon, but terminal genomic DNA were not. A srthfic digoxigenin (DIG )—lake]ed gole was gepraal from a 13 1dlolse-ir-clonerl fragment, Six EDSV Taiwan isolates were dettd by the nucleic acid pde methcwi, but one avian adenovirus (NO.9 stiain) was not. The 1W pg volume of EDSV DNA and infected duck enhyonanl fihtblast (DEF) cells were surveyed by southern blotting and DNA hy&idization. The esblishal DNA obu ezhnique of this study can ke used as a detective tool for infection of EDSV.

Keyword

Egg drop syndrome, Nucleic acid prolt