Home
 /  Publication  /  Volum 41  /  Publication Development of Isolation Techniques on Koi Herpesvirus
Publication Development of Isolation Techniques on Koi Herpesvirus  

Update Date: [2013-11-14]

NO.: 41
 
Reportno.

AHRI report No.41

Topic

Development of Isolation Techniques on Koi Herpesvirus

 

Department 1. Animal Health Research bstitrt Ouncil ofAgriculture, Executive Yuan Depax-tinent 
2.National Taiwan Unitity Derfrnent of Veterinary Melicine
3.Fishery Research Institutes Bioe±nology Division
Author Th, C, SM Huang, C (Therng WM Chang and PH Chao
Summary

The pirpcse of this prozt is establishing cell lines permissible to koi herpes’’ixus (KHV) for developing the isolation methcd of KHV. We established two primary cell lines from koi (35-passage koi gill and koi fin); the optimum growth medium was either IAlrovita- 15 supplementl with 10% fetal calf serum cii a mixture containing 60% Earle’ s minimum essential medium, 30% Leiboviiz-15, 10% h-yptose phcepha and 10% fetal calf serum. The optimum growth mperature was Iretween 20-25cC, Thc€e two cell lines were susceptible to KHV and the cytopathic effect (CPE) appeared inkoigillcelll0-14 days postinfection;and20days pDGtinfectioninfmcell. The typical (YE showed cytoplasmic vuolation and cell lysis. The (YE apreaxel only at first generation of isolation but it disappeared after the sub-passage of the virus in the sarnecefls.

Keyword

Koi Isolation; Koi herpesvirus